Journal: Fungal biology

Article Title: Morphological changes in melanized and non-melanized Cryptococcus neoformans cells post exposure to sparsely and densely ionizing radiation demonstrate protective effect of melanin.

doi: 10.1016/j.funbio.2017.08.010

Figure Lengend Snippet: Fig. 5. Micrographs of CN cells obtained with fluorescence microscopy after treatment with maximum doses of alpha particle or deuteron irradiation relative to sham-irradiated. Following exposure cells were fixed with formaldehyde prior to staining with propidium iodide and digestion with RNAse to specifically stain the DNA.

Article Snippet: Samples were then transferred into a propidium iodide/RNAse A solution (Novus, Littleton, CO) and stained for at least 30 min prior to imaging.

Techniques: Microscopy, Irradiation, Staining

Journal: Cell Death Discovery

Article Title: WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopment

doi: 10.1038/s41420-026-02977-4

Figure Lengend Snippet: A Scheme of WWP1 variant-expressing cell line establishment and downstream analysis. The image is created with Biorender.com. B Representative images of dead cell population in WWP1 variants-expressing HeLa cell lines treated with gefitinib. Red, propidium iodide (PI). Scale bar, 200 μm. C Quantification of the PI + counts in HeLa cell lines treated with gefitinib. n = 3 for each condition. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05; ** p < 0.01. D Functional GO enrichment analysis of upregulated and downregulated DEGs between WWP1 E798V and control cell lines. The bar color indicates the cluster categories of the GO term. BP, biological process; CC, cellular component. E Differential PROGENy pathway activity scores of WWP1 E798V relative to control. The pathway with the lowest value, TGFβ, is indicated by a deep blue color. F Gene set enrichment plots for TGFβ signaling and epithelial-mesenchymal transition. NES, normalized enrichment score. G Western blot analysis of phospho-SMAD2 and SMAD2 in WWP1 variant-expressing HeLa cell lines treated with vehicle or hTGFβ1 (10 ng/mL). ACTIN is used as the loading control. H Quantification of normalized pSMAD2 to SMAD2 protein expression ratio in WWP1 variant-expressing HeLa cell lines. n = 5. Kruskal-Wallis test with Dunn’s post hoc test. * p < 0.05; ns not significant. Bar graphs indicate mean ± SEM.

Article Snippet: Non-cytotoxic dosing conditions were determined by the cell viability using Propidium Iodide/RNase staining solution (Cell Signaling Technology, #4087).

Techniques: Variant Assay, Expressing, Functional Assay, Control, Activity Assay, Western Blot

Journal: Microarrays

Article Title: Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

doi: 10.3390/microarrays5020008

Figure Lengend Snippet: Representative time traces of fluorescent signals monitored in the polarity Sensor for Viability and Apoptosis (pSIVA) ( A ) and propidium iodide (PI) ( B ) channels. A few typical traces are highlighted in green and red, respectively. The first apoptotic events are observed after 3 h whereas the majority of induced cell deaths occur after 20 h. The decrease in the pSIVA signal at late times can be attributed to bleaching of the fluorophores. Fluorescent signals are background corrected.

Article Snippet: Cells were stained with polarity Sensor for Viability and Apoptosis (pSIVA-IANBD, Novus Biologicals) [ , ] (the stock solution was diluted 1:50) and 1 μ M propidium iodide (Novus Biologicals).

Techniques: