Journal: Neurotherapeutics

Article Title: CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice

doi: 10.1007/s13311-019-00737-0

Figure Lengend Snippet: EFV effects on amyloid pathology. (a) The content of soluble and insoluble amyloid β (Aβ) peptides in the brain homogenates of 5XFAD mice. The quantifications were carried out by ELISA. The results are presented as means ± SD of measurements in individual mice. Both female (n = 5 per treatment group, pink rectangles) and male (n = 4 per treatment group, blue rectangles) mice were used. No significant differences were found between control (Cntr) and EFV-treated (Tx) mice when female and male mice were analyzed as one group or separately. (b and d) Representative images (n = 5 male mice per treatment group) of the brain sections stained with 6E10 and Thioflavin S, respectively. Nuclei were stained with DAPI (b) or propidium iodide (d, false colored in blue). (c and e) Quantifications of the 6E10- and Thioflavin S-positive plagues. Area of the plaques is expressed as a percentage from the total area of either cortex or hippocampus. *P ≤ 0.05 by an unpainted, two-tailed Student’s t test. Cntr, control mice; Tx, EFV-treated mice. Scale bars, 150 μm

Article Snippet: Slides were mounted either with ProLong Gold Antifade reagent with DAPI ( {"type":"entrez-protein","attrs":{"text":"P36935","term_id":"549826","term_text":"P36935"}} P36935 , Thermo Fisher Scientific, Waltham, MA) or with VECTASHIELD Antifade Medium with Propidium Iodide (H-1300-10; Vector Laboratories, Inc., Burlingame, CA).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test

Journal: PLOS One

Article Title: A novel peptide mimetic, brilacidin, for combating multidrug-resistant Neisseria gonorrhoeae

doi: 10.1371/journal.pone.0325722

Figure Lengend Snippet: A) Propidium iodide fluorescence after treating N. gonorrhoeae with either azithromycin or brilacidin to predict the permeabilization of the cytoplasmic membrane. B) Percentage of ATP leakage from N. gonorrhoeae treated with brilacidin or azithromycin relative to Triton X-100 (positive control with complete ATP leakage). Asterisks denote statistically significant differences between test agents and DMSO (untreated), * (P < 0.05), ** (P < 0.01), and **** (P < 0.0001) as determined by one-way ANOVA.

Article Snippet: Media and reagents including McCoy’s 5A medium and hematin (Sigma Aldrich, St. Louis, MO, USA), triton X-100 (Acros Organics, Fair Lawn, NJ, USA), BacTiter-Glo reagent (Promega Corporation, Madison, WI, USA), Propidium iodide (PI) and nicotinamide adenine dinucleotide (NAD) (Chem-Impex International, Wood Dale, IL, USA), MTS (3-(4,5-dimethylthia- zol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (Abcam, Waltham, MA, USA), and brucella broth, chocolate II agar plates, IsoVitaleX and bovine hemoglobin (Becton, Dickinson and Company, Cockeysville, MD, USA), were obtained from chemical vendors.

Techniques: Fluorescence, Membrane, Positive Control