propidium iodide Search Results


propidium iodide  (Miltenyi Biotec)
97
Miltenyi Biotec propidium iodide
Propidium Iodide, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti erg  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology rabbit anti erg
Rabbit Anti Erg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide  (Elabscience Biotechnology)
95
Elabscience Biotechnology propidium iodide
Propidium Iodide, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide  (Thermo Fisher)
99
Thermo Fisher propidium iodide
Propidium Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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om 010 propidium iodide tocris  (Tocris)
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Tocris om 010 propidium iodide tocris
Om 010 Propidium Iodide Tocris, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry kit  (Danaher Inc)
99
Danaher Inc flow cytometry kit
Flow Cytometry Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmso selleckchem s2742 propidium iodide  (Selleck Chemicals)
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Selleck Chemicals dmso selleckchem s2742 propidium iodide
Dmso Selleckchem S2742 Propidium Iodide, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide pi  (Novus Biologicals)
91
Novus Biologicals propidium iodide pi
Propidium Iodide Pi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide pi staining  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology propidium iodide pi staining
Propidium Iodide Pi Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide pi kit  (R&D Systems)
91
R&D Systems propidium iodide pi kit
Human and rabbit complement-dependent cytotoxicity in human astrocytes. A) LDH assay results. In astrocyte-culture supernatants with non-heated complement after purified IgG treatment, LDH release was significantly higher compared to those by heat-inactivated complement or normal media alone. Moreover, LDH release in the supernatants with non-heated rabbit complement was significantly higher than that with non-heated human complement. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. B) Results of cellular nuclear size. After 60-minute treatment with human or rabbit complement, astrocytic nuclear size pre-treated with NMO’s purified IgG was significantly larger compared with other conditions. In addition, there was a significant difference in nuclear size between non-heated rabbit and human complements. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. C) Time-course of <t>propidium</t> iodide (PI) positivity. PI assays showed a significant increase in the percentage of positive cells after exposure to AQP4-antibody (NMO-IgG) and non-heated rabbit complement compared with non-heated human complement.
Propidium Iodide Pi Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide pi staining  (ImmunoChemistry Technologies)
90
ImmunoChemistry Technologies propidium iodide pi staining
Human and rabbit complement-dependent cytotoxicity in human astrocytes. A) LDH assay results. In astrocyte-culture supernatants with non-heated complement after purified IgG treatment, LDH release was significantly higher compared to those by heat-inactivated complement or normal media alone. Moreover, LDH release in the supernatants with non-heated rabbit complement was significantly higher than that with non-heated human complement. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. B) Results of cellular nuclear size. After 60-minute treatment with human or rabbit complement, astrocytic nuclear size pre-treated with NMO’s purified IgG was significantly larger compared with other conditions. In addition, there was a significant difference in nuclear size between non-heated rabbit and human complements. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. C) Time-course of <t>propidium</t> iodide (PI) positivity. PI assays showed a significant increase in the percentage of positive cells after exposure to AQP4-antibody (NMO-IgG) and non-heated rabbit complement compared with non-heated human complement.
Propidium Iodide Pi Staining, supplied by ImmunoChemistry Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide staining solution  (SouthernBiotech)
93
SouthernBiotech propidium iodide staining solution
Human and rabbit complement-dependent cytotoxicity in human astrocytes. A) LDH assay results. In astrocyte-culture supernatants with non-heated complement after purified IgG treatment, LDH release was significantly higher compared to those by heat-inactivated complement or normal media alone. Moreover, LDH release in the supernatants with non-heated rabbit complement was significantly higher than that with non-heated human complement. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. B) Results of cellular nuclear size. After 60-minute treatment with human or rabbit complement, astrocytic nuclear size pre-treated with NMO’s purified IgG was significantly larger compared with other conditions. In addition, there was a significant difference in nuclear size between non-heated rabbit and human complements. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. C) Time-course of <t>propidium</t> iodide (PI) positivity. PI assays showed a significant increase in the percentage of positive cells after exposure to AQP4-antibody (NMO-IgG) and non-heated rabbit complement compared with non-heated human complement.
Propidium Iodide Staining Solution, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Human and rabbit complement-dependent cytotoxicity in human astrocytes. A) LDH assay results. In astrocyte-culture supernatants with non-heated complement after purified IgG treatment, LDH release was significantly higher compared to those by heat-inactivated complement or normal media alone. Moreover, LDH release in the supernatants with non-heated rabbit complement was significantly higher than that with non-heated human complement. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. B) Results of cellular nuclear size. After 60-minute treatment with human or rabbit complement, astrocytic nuclear size pre-treated with NMO’s purified IgG was significantly larger compared with other conditions. In addition, there was a significant difference in nuclear size between non-heated rabbit and human complements. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. C) Time-course of propidium iodide (PI) positivity. PI assays showed a significant increase in the percentage of positive cells after exposure to AQP4-antibody (NMO-IgG) and non-heated rabbit complement compared with non-heated human complement.

Journal: Biochemistry and Biophysics Reports

Article Title: Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica

doi: 10.1016/j.bbrep.2016.05.012

Figure Lengend Snippet: Human and rabbit complement-dependent cytotoxicity in human astrocytes. A) LDH assay results. In astrocyte-culture supernatants with non-heated complement after purified IgG treatment, LDH release was significantly higher compared to those by heat-inactivated complement or normal media alone. Moreover, LDH release in the supernatants with non-heated rabbit complement was significantly higher than that with non-heated human complement. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. B) Results of cellular nuclear size. After 60-minute treatment with human or rabbit complement, astrocytic nuclear size pre-treated with NMO’s purified IgG was significantly larger compared with other conditions. In addition, there was a significant difference in nuclear size between non-heated rabbit and human complements. C: non-heated complement, HIC: Heat-inactivated complement,*: P<0.0001. C) Time-course of propidium iodide (PI) positivity. PI assays showed a significant increase in the percentage of positive cells after exposure to AQP4-antibody (NMO-IgG) and non-heated rabbit complement compared with non-heated human complement.

Article Snippet: Cellular cytotoxicity with or without apoptosis was analyzed using a propidium iodide (PI) kit (TACS Annexin V-FITC, R&D Systems, Minneapolis, USA).

Techniques: Lactate Dehydrogenase Assay, Purification

Results of the cytotoxic assay. A) Results of immunostaining and western blotting of complement regulatory protein in the astrocyte. CD55 and CD59 were observed by immunostaining of cultured human astrocytes and western blotting of the membrane fraction. In addition, CD35 and CD46 on the cultured astrocyte were not detected in the immunostaining and western blotting. B) Results of propidium iodide (PI) positivity at 60 min after complement treatment. PI assays showed a significant increase in the percentage of PI-positive cells after CD55-siRNAs transfection compared with non-siRNA treated astrocyte. Transfection of CD59-siRNAs showed a limited effect on PI positivity. PI positivity after the transfection of CD55 and CD59 with human complement showed no significant difference against astrocytes with rabbit complement.

Journal: Biochemistry and Biophysics Reports

Article Title: Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica

doi: 10.1016/j.bbrep.2016.05.012

Figure Lengend Snippet: Results of the cytotoxic assay. A) Results of immunostaining and western blotting of complement regulatory protein in the astrocyte. CD55 and CD59 were observed by immunostaining of cultured human astrocytes and western blotting of the membrane fraction. In addition, CD35 and CD46 on the cultured astrocyte were not detected in the immunostaining and western blotting. B) Results of propidium iodide (PI) positivity at 60 min after complement treatment. PI assays showed a significant increase in the percentage of PI-positive cells after CD55-siRNAs transfection compared with non-siRNA treated astrocyte. Transfection of CD59-siRNAs showed a limited effect on PI positivity. PI positivity after the transfection of CD55 and CD59 with human complement showed no significant difference against astrocytes with rabbit complement.

Article Snippet: Cellular cytotoxicity with or without apoptosis was analyzed using a propidium iodide (PI) kit (TACS Annexin V-FITC, R&D Systems, Minneapolis, USA).

Techniques: Immunostaining, Western Blot, Cell Culture, Membrane, Transfection